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Exam 20: DNA Technology and Genetic Engineering

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Question 31

True/False

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Producing transgenic animals is much easier than generating transgenic plants.

A) True
B) False

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Question 32

Multiple Choice

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The following are steps involved in producing recombinant DNA using human DNA and a plasmid. Which one of the following answers best reflects the proper order of these steps? 1. select bacteria that contain human DNA 2) isolate and purify DNA to be recombined 3) add DNA ligase to join human DNA with plasmid 4) cut human DNA and plasmid with restriction enzyme 5) introduce new plasmid to bacteria

A) 4, 1, 5, 3, 2
B) 2, 4, 3, 5, 1
C) 2, 5, 1, 3, 4
D) 4, 3, 2, 1, 5
E) 5, 1, 2, 4, 3

F) D) and E)
G) None of the above

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Question 33

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A small amount of DNA was located at a crime scene. Which one of the following techniques is most likely to be used to amplify the DNA so it can be identified more accurately?

Small pieces of DNA can be amplified by a process known as polymerase chain reaction.

The goal of polymerase chain reaction (PCR) is to determine the sequence of nucleotides in a stretch of DNA.

During polymerase chain reaction, DNA amplification comes to an end

The first successful treatment of a genetic disorder by gene therapy was the curing of a 4-year-old girl who had severe combined immunodeficiency disease. Because the engineered cells do not live forever, this child must supplement her treatment with regular doses of

In recombinant DNA technology, DNA is cut at specific nucleotide sequences by ________ enzymes.

Short single-stranded pieces of DNA called ________ attach to a DNA template to serve as the beginning site of DNA replication.

Polymerase chain reaction (PCR) is useful for amplifying a small piece of DNA but is not a good technique for cloning genes because

Organisms that can contain genes introduced from other species are referred to as

Some genetically engineered tomato plants produce tomatoes that last longer on the grocery shelf; some genetically engineered cotton plants are resistant to insects.

Insertion of a gene into another organism's DNA so that it can be transferred to another organism like a bacterium is called

Bacterial ________ DNA can be easily removed from the bacterium, modified by adding a gene of interest, and then reinserted into the bacterium to be replicated.

During PCR, heating of DNA is required to

________ is a useful technique in criminal investigations because humans contain many copies of repeating or junk sequences, the length of which can be unique to an individual.

The insertion of human genes into human cells to treat a disease is known as ________.

Gene therapy is currently under investigation as a possible way to cure cystic fibrosis. How is the viral vector carrying the normal gene delivered to the affected cells?

A DNA sequence that can be read the same backward and forward is called a(n)

When DNA fragments from different sources are mixed together, they begin combining with each other due to